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Firdausy Kurnia Maulana ¹, Didik Handijatno ², Hani Plumeriastuti ³, Rahaju Ernawati ², Wiwiek Tyasningsih ², Mufasirin ⁴

Affiliations
1 Airlangga University, ID
2 Department of Microbiology and Mycology, Airlangga University, ID
3 Department of Veterinary Pathology, Airlangga University, ID
4 Department of Veterinary Parasitology, Airlangga University, ID

Abstract

Haemorrhagic Septicaemia (HS), in Indonesia known as Ngorok disease is a fatal acute septicemia disease in cattle and buffalo caused by Pasteurella multocida. Outer membrane Protein H (OmpH) is one of the major proteins in P. multocida envelope has been purified and characterized as porin immunodominant. The aim of this study is to find out the homology of nucleotide sequence of 37 kDa OmpH gene of P. multocida of local isolate from NTT with vaccine strain (Katha) and 5 referen isolates and predict the epitope of 37 kDa OmpH gene. Detection of 37 kDa OmpH gene was done using PCR. Homology analysis of nucleotide and amino acid sequences was performed on the BLAST program at NCBI. Epitope prediction of 37 kDa OmpH genes P. multocida from NTT and vaccine strains was performed using the online programme B Cell Epitope Prediction Tools. The results showed that 37 kDa OmpH gene of P. multocida from NTT has 99% homology to vaccine strain (Katha) and isolates from China, Iran, India, Mesir, and 89% homology to isolate from USA and 37 kDa OmpH gene of P. multocida local isolate from NTT were likely to be immunogen candidates based on B cell epitope that had 11 epitopes. These findings indicate that P. multocida local isolate from NTT has a chance as immunogen candidate, which can be developed as a vaccine candidate and diagnostic kit for HS.

Keywords

Haemorrhagic Septicaemia, P. Multocidatype B, Outer Membrane Protein H(omp H) Gene, Pcr, Sequence, Epitope.

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Franch Dalahi ¹, Silvia Yuhana ¹, Ketut Mahardika ², Zafran ², Indah Mastuti ², Mufasirin ³, Hari Suprapto ⁴

Affiliations
1 Biotechnology of Fisheres and Marine, Pasca Sarjana, Airlangga University, ID
2 Department of Parasitology, Center for Marine Aquaculture Research and Fisheries Extension, ID
3 Department of Parasitology, Airlangga University,, ID
4 Department of Fish Health, Airlangga University, ID

Abstract

Grouper cultivation is still constrained by the availability of seeds due to diseases caused by bacteria. Vibriosis is a disease caused by bacterial infections of the Vibrio genus such as Vibrio alginolyticus and Vibrio parahaemolyticus which are the most common in hatcheries. Whole Cell Vaccine (WCV) contains endotoxin components as antigens to stimulate antibody formation, including lipopolysaccharide from the cell wall of gram-negative bacteria. There is a problem of oral vaccination which can damagewhen it enters the low pH digestive system. Therefore, it is necessary to conduct a research to find out the ability of the Whole cell vaccine (WCV) of Vibrio alginoliticus bacteria by microencapsulation method of alginate gel as an oral alginate particle vaccine Vibrio alginoliticus to increase the survival of the grouper seeds of Epinephelus sp. This study used four types of treatment, namely control (-) without vaccine, control (+) using alginate only, dosage treatment 0.025 mg/5gr BB fish/day and 0.05 mg/5gr BB fish/day. The results of the Whole Cell Vaccine using alginate gel microcapsules process can increase the life range of cantang grouper seeds by 80% at 0.05 mg/5gr BB fish/day and 73% at 0.025 mg/5gr BB fish/day.

Keywords

Vibrio Alginolyticus, Whole Cell Vaccine, Alginate, Grouper, Vibriosis.

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Muhammad Rama Imam Saputra ¹, April H. Wardhana ², Mufasirin ³, Setiawan Koesdarto ³, Lucia Tri Suwanti ³, Nunuk Dyah Retno Lastuti ³, Endang Suprihati ³

Affiliations
1 Disease and Veteriner Public Health Science, Airlangga University, ID
2 Department of Parasitology, Veterinery Center of Bogor, ID
3 Department of Parasitology, Airlangga University, ID

Abstract

Trypanosomiasis or surra is a disease caused by blood parasite of Trypanosoma evansi. Trypanosomiasis disease in Indonesia includes Strategic Diseases of Infectious Diseases (SDID). The purpose of this study is to detect Trypanosomiasis in horses in Bima, Indonesia. A total of 90 horse blood samples were used for the study. Sample examination using blood smears technique with Giemsa staining and Card Agglutination Test of Trypanosoma evansi (CATT). The result of examination of blood samples by microscope obtained 3 positive samples infected T. evansi and by CATT obtained 1 positive samples containing antibodies against T. evansi. It was concluded that trypanosomiasis in horses in Bima was 3.3%.

Keywords

Bima, Blood Smears, Card Agglutination Test Of Trypanosoma Evansi (CATT), Horse, Trypanosoma Evansi.

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